- Bo Burnham (2,152,777 followers) comedy
- Tenacious D (2,001,292 followers) comedy
- The Lonely Island (1,139,062 followers) comedy
- "Weird Al" Yankovic (1,107,159 followers) comedy, polka
- Joe Rogan (819,248 followers) comedy, spoken word
- Ninja Sex Party (429,655 followers) comedy
- Epic Rap Battles of History (421,703 followers) comedy
- Flight of the Conchords (395,402 followers) comedy
- Franco Escamilla (371,384 followers) comedy, trova
- Richard Cheese (351,560 followers) comedy, swing music
- Bill Burr (308,484 followers) comedy
- Rucka Rucka Ali (306,076 followers) comedy
- Hugh Laurie (280,350 followers) comedy
- Starbomb (270,459 followers) comedy
- Your Favorite Martian (270,058 followers) comedy
- Jon Lajoie (251,774 followers) comedy
- Kevin Hart (251,143 followers) comedy
- John Mulaney (217,427 followers) comedy
- Jim Gaffigan (205,938 followers) comedy
- Joe Rogan (205,928 followers) comedy
- Adam Sandler (205,483 followers) comedy
- Louis C.K. (182,689 followers) comedy
- The Divine Comedy (175,390 followers) baroque pop, britpop, comedy
- Robin Williams (169,311 followers) comedy
- Tim Minchin (163,594 followers) comedy
- Gabriel Iglesias (163,337 followers) comedy
- George Carlin (151,276 followers) comedy, spoken word
- Theo Von (149,696 followers) comedy
- Monty Python (145,823 followers) comedy, spoken word
- Rodney Carrington (140,119 followers) comedy
- Mitch Hedberg (135,903 followers) comedy, spoken word
- Daniel Tosh (133,039 followers) comedy
- Tom Lehrer (131,661 followers) comedy
- Stephen Lynch (130,920 followers) comedy
- Tom Segura (129,087 followers) comedy
- Eddie Murphy (126,124 followers) comedy
- Chris Rock (122,188 followers) comedy
- Jonathan Coulton (115,286 followers) comedy
- Garfunkel and Oates (114,396 followers) comedy
- Dane Cook (110,406 followers) comedy
- Dave Chappelle (109,456 followers) comedy
- Patton Oswalt (106,956 followers) comedy, spoken word
- Brian Regan (105,391 followers) comedy
- Nate Bargatze (105,347 followers) comedy
- Ray Stevens (104,211 followers) comedy, classic country
- Torsten Sträter (99,997 followers) comedy
- Ron White (98,836 followers) comedy
- Jeff Dunham (95,655 followers) comedy
- Lewis Black (90,891 followers) comedy, spoken word
- Kevin Bloody Wilson (90,102 followers) comedy
- Richard Pryor (87,655 followers) comedy, spoken word
- Da Vinci's Notebook (86,401 followers) sea shanties, comedy
- Katt Williams (84,411 followers) comedy
- Les Luthiers (83,912 followers) comedy, folklore argentino
- Henry Rollins (82,977 followers) spoken word, comedy
- Hannibal Buress (82,255 followers) comedy
- Anthony Jeselnik (79,526 followers) comedy
- Reggie Watts (78,862 followers) comedy, jazz house
- Cheech & Chong (74,783 followers) comedy
- Matt Rife (73,616 followers) comedy
- Shane Gillis (70,978 followers) comedy
- Bert Kreischer (69,123 followers) comedy
- Mike Birbiglia (67,523 followers) comedy
- Bill Engvall (66,557 followers) comedy
- George Lopez (65,160 followers) comedy
- Steve Martin (65,131 followers) bluegrass, comedy, newgrass
- Larry The Cable Guy (64,901 followers) comedy
- Jeff Foxworthy (64,602 followers) comedy
- Polo Polo (62,707 followers) comedy
- Bill Hicks (60,856 followers) comedy, spoken word
- Spinal Tap (59,990 followers) comedy
- Nick Swardson (59,708 followers) comedy
- Felix Lobrecht (59,451 followers) comedy
- Norm MacDonald (54,440 followers) comedy
- Jim Jefferies (53,158 followers) comedy
- Tim Hawkins (52,906 followers) comedy
- Amy Schumer (50,914 followers) comedy
- Marc-Uwe Kling (50,376 followers) comedy
- Steven Wright (50,021 followers) comedy, spoken word
- Ricky Gervais (49,637 followers) comedy
- Steve Harvey (49,455 followers) comedy
- Jimmy Carr (47,957 followers) comedy
- The Axis of Awesome (47,018 followers) comedy
- Christopher Titus (46,965 followers) comedy
- Doug Stanhope (46,347 followers) comedy, spoken word
- Mark Lowry (46,195 followers) southern gospel, comedy, country christian, hymns, gospel, christian alternative rock
- Kyle Kinane (45,153 followers) comedy
- Jochem Myjer (45,072 followers) comedy
- Dave Attell (44,659 followers) comedy
- Jürgen von der Lippe (42,693 followers) comedy, schlager, neue deutsche welle
- Dieter Nuhr (42,317 followers) comedy
- Chad Daniels (41,588 followers) comedy
- The Midnight Beast (41,064 followers) comedy
- David Cross (40,584 followers) comedy
- Denis Leary (40,171 followers) comedy
- Marc Maron (38,937 followers) comedy
- Urbanus (37,881 followers) comedy, nederpop
- Goldie Lookin Chain (37,799 followers) comedy
- Kurt Krömer (37,282 followers) comedy
- Cledus T. Judd (37,094 followers) comedy