- Bo Burnham (2,108,662 followers) comedy
- Tenacious D (1,956,548 followers) comedy
- The Lonely Island (1,117,889 followers) comedy
- "Weird Al" Yankovic (1,058,745 followers) comedy, polka
- Joe Rogan (821,125 followers) comedy, spoken word
- Ninja Sex Party (424,421 followers) comedy
- Epic Rap Battles of History (416,346 followers) comedy
- Flight of the Conchords (387,745 followers) comedy
- Franco Escamilla (359,201 followers) comedy, trova
- Richard Cheese (343,538 followers) comedy, swing music
- Bill Burr (306,502 followers) comedy
- Rucka Rucka Ali (294,066 followers) comedy
- Hugh Laurie (273,999 followers) blues, comedy
- Starbomb (268,363 followers) comedy
- Your Favorite Martian (263,565 followers) comedy
- Kevin Hart (248,403 followers) comedy
- Jon Lajoie (239,884 followers) comedy
- John Mulaney (215,447 followers) comedy
- Jim Gaffigan (203,855 followers) comedy
- Joe Rogan (198,581 followers) comedy
- Adam Sandler (197,381 followers) comedy
- Louis C.K. (180,364 followers) comedy
- The Divine Comedy (169,685 followers) baroque pop, britpop, comedy
- Robin Williams (166,816 followers) comedy
- Tim Minchin (161,053 followers) comedy
- Gabriel Iglesias (159,956 followers) comedy
- George Carlin (149,494 followers) comedy, spoken word
- Monty Python (141,963 followers) comedy, spoken word
- Theo Von (139,958 followers) comedy
- Rodney Carrington (136,227 followers) comedy
- Mitch Hedberg (134,696 followers) comedy, spoken word
- Daniel Tosh (131,940 followers) comedy
- Stephen Lynch (129,308 followers) comedy
- Tom Lehrer (125,358 followers) comedy
- Tom Segura (124,860 followers) comedy
- Chris Rock (121,764 followers) comedy
- Eddie Murphy (115,979 followers) comedy
- Jonathan Coulton (113,420 followers) comedy
- Garfunkel and Oates (112,030 followers) comedy
- Dane Cook (109,579 followers) comedy
- Dave Chappelle (108,087 followers) comedy
- Patton Oswalt (106,098 followers) comedy, spoken word
- Brian Regan (103,831 followers) comedy
- Ray Stevens (100,416 followers) comedy, classic country
- Nate Bargatze (99,742 followers) comedy
- Ron White (98,082 followers) comedy
- Torsten Sträter (97,953 followers) comedy
- Jeff Dunham (94,304 followers) comedy
- Lewis Black (90,619 followers) comedy, spoken word
- Kevin Bloody Wilson (86,476 followers) comedy
- Richard Pryor (86,359 followers) comedy, spoken word
- Da Vinci's Notebook (84,423 followers) sea shanties, comedy
- Katt Williams (82,351 followers) comedy
- Les Luthiers (82,297 followers) comedy, folklore argentino
- Hannibal Buress (81,889 followers) comedy
- Henry Rollins (80,886 followers) spoken word, comedy
- Anthony Jeselnik (77,880 followers) comedy
- Reggie Watts (77,751 followers) comedy, jazz house
- Cheech & Chong (71,375 followers) comedy
- Matt Rife (67,974 followers) comedy
- Bert Kreischer (67,736 followers) comedy
- Mike Birbiglia (66,889 followers) comedy
- Bill Engvall (65,837 followers) comedy
- Shane Gillis (65,411 followers) comedy
- George Lopez (64,766 followers) comedy
- Larry The Cable Guy (63,939 followers) comedy
- Jeff Foxworthy (63,448 followers) comedy
- Steve Martin (62,921 followers) bluegrass, comedy, newgrass
- Polo Polo (62,402 followers) comedy
- Bill Hicks (60,187 followers) comedy, spoken word
- Nick Swardson (59,241 followers) comedy
- Felix Lobrecht (58,876 followers) comedy
- Spinal Tap (57,868 followers) comedy
- Norm MacDonald (53,177 followers) comedy
- Jim Jefferies (52,122 followers) comedy
- Tim Hawkins (51,110 followers) comedy
- Amy Schumer (50,829 followers) comedy
- Steven Wright (49,657 followers) comedy, spoken word
- Steve Harvey (48,676 followers) comedy
- Ricky Gervais (48,225 followers) comedy
- Christopher Titus (46,544 followers) comedy
- The Axis of Awesome (46,045 followers) comedy
- Doug Stanhope (45,956 followers) comedy, spoken word
- Mark Lowry (45,343 followers) southern gospel, comedy, country christian, hymns, gospel, christian
- Kyle Kinane (44,205 followers) comedy
- Jimmy Carr (44,202 followers) comedy
- Dave Attell (44,121 followers) comedy
- Jochem Myjer (43,632 followers) comedy
- Jürgen von der Lippe (42,158 followers) comedy, schlager, neue deutsche welle
- Dieter Nuhr (42,072 followers) comedy
- The Midnight Beast (40,647 followers) comedy
- Chad Daniels (40,599 followers) comedy
- David Cross (40,221 followers) comedy
- Marc Maron (38,347 followers) comedy
- Denis Leary (38,146 followers) comedy
- Urbanus (37,388 followers) comedy, nederpop
- Kurt Krömer (37,096 followers) comedy
- Maria Bamford (36,368 followers) comedy
- John Pinette (36,247 followers) comedy
- Goldie Lookin Chain (36,030 followers) comedy